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User Guide | 2 1
2 Gel Doc XR+ Imager
Refer to the Gel Doc™ XR+ Installation Guide found in your Gel Doc XR+ Installation
Kit for information about installing and calibrating your system. Refer to
Chapter 4,
Setting Up the Instrument with Image Lab Software for information about installing
Image Lab™ software.
To recalibrate your system because you have acquired new accessories, refer to
Chapter 11, System Calibration.
Gel Doc XR+ Imager Workflow
Following are the basic steps to acquiring, analyzing, and archiving an image using
the Gel Doc XR+ system and Image Lab software:
1. Select an existing protocol or customize a new one.
2. Position the sample to be imaged.
3. Run a selected protocol.
4. View the displayed results.
5. Optimize the analysis.
6. Generate a report.
7. Save or export the results.
- Gel Doc™ XR+ and 1
- ChemiDoc™ 1
- Systems with Image Lab™ 1
- Software 1
- Table of Contents 3
- User Guide 5
- User Guide 7
- User Guide 9
- 10
- Safety and Regulatory 11
- Compliance 11
- Warranty 12
- General Precautions 12
- Regulatory Notices 13
- Instrument Safety Warnings 13
- Power Safety Information 15
- 1 Introduction 17
- ChemiDoc XRS+ Imaging System 18
- System Components 18
- Universal Hood 19
- Image Lab Software 19
- Optional Accessories 20
- 2 Gel Doc XR+ Imager 21
- Gel Doc XR+ Applications 22
- User Guide 23
- 2 24
- 3 ChemiDoc XRS+ Imager 25
- ChemiDoc XRS+ Applications 26
- User Guide 27
- 3 28
- 4 Setting Up the Instrument 29
- Installing Image Lab Software 30
- User Guide 31
- User Guide 33
- User Guide 35
- To install Image Lab on a Mac 36
- User Guide 37
- User Guide 39
- 3. Click Activate 40
- User Guide 41
- User Guide 43
- User Guide 45
- To enable secure mode 47
- To disable secure mode 48
- User Guide 49
- Setting Security Preferences 50
- User Guide 51
- Renaming Security Groups 53
- Changing Security Preferences 54
- 5 Image Lab Software 55
- Overview 55
- Protocols .ptl .sptl 56
- Images .scn .sscn 56
- File type File Extension Icon 56
- Interface Overview 57
- Main Toolbar 58
- Results Data 58
- Display Toolbox 58
- Start Page 59
- Analysis Tool Box 59
- Status Bar 61
- Menu Commands 62
- Interface Overview 63
- User Guide 63
- Edit Menu Commands 64
- View Menu Commands 65
- Security Menu Commands 66
- Window Menu Commands 66
- Help Menu Commands 67
- 6 Acquiring Images 69
- The Protocol Setup Window 70
- Creating a Protocol 71
- Setting Up a New Protocol 72
- User Guide 73
- 6 74
- User Guide 75
- User Guide 77
- User Guide 79
- User Guide 81
- Step 4. Specify Output 82
- Review Protocol Settings 83
- Editing a Saved Protocol 85
- Positioning the Gel 87
- Running a Protocol 88
- User Guide 89
- To save a single SAM image 90
- To save all SAM images 90
- User Guide 91
- Application Tables 94
- User Guide 95
- 7 Viewing Images 97
- Displaying Gel Images 98
- Zoom Tools 99
- Fit in Window 100
- Image Transform 100
- Options: 101
- Image Colors 102
- 3-D Projection 103
- Displaying Data 104
- Analysis Table Options 105
- 7 106
- User Guide 107
- Background 109
- Scale to Fit Graph 110
- Include Background 110
- Identify Bands by 110
- Adjusting Band Boundaries 111
- Standard Curve 112
- User Guide 113
- 8 Analyzing Images 115
- Auto Detection Settings 116
- Up arrow 117
- Image Tools 118
- Correcting a Slanted Gel 119
- Cropping a Gel Image 119
- Inverting Data 120
- Merging Images 120
- Lane and Bands Tools 121
- Detecting Lanes 122
- Single Lane Tools 123
- Lane Background Subtraction 124
- Copying Lanes 125
- Detecting Bands 126
- User Guide 127
- Advanced Detection Options 128
- Editing the Detected Bands 129
- 8 130
- User Guide 131
- Molecular Weight Standard 132
- Standard Lanes 133
- Quantity Tools 135
- Absolute Quantity Tab 137
- User Guide 139
- Annotation Tools 141
- Add Annotations 142
- Alignment 142
- Text Properties 143
- Volume Tools 144
- User Guide 145
- Volume Types 146
- Volume Background Subtraction 147
- Relative Volume Quantity 148
- Absolute Volume Quantity 148
- Regression Methods 149
- 9 Generating Reports 151
- Report Settings 152
- User Guide 153
- Lane and Band Table Tab 154
- Print Report 155
- Print Report to a PDF File 155
- Adjust the Printer Settings 156
- 10 Exporting Results 157
- 10 158
- In this dialog box you can: 159
- Screenshot Tool Export 161
- Analysis Table Export 161
- 11 System Calibration 163
- Recalibrating Your Imager 164
- User Guide 165
- 11 166
- 12 Image Lab Logs 167
- Viewing the Instrument Log 168
- Viewing the System Log 169
- Viewing the Document Log 170
- Displaying Log Data 171
- Filtering Data in Logs 172
- Setting filters in logs 173
- Removing filters in logs 174
- Exporting Logs 175
- Copy log entries to clipboard 176
- Export log entries to File 176
- Export log entries to Excel 176
- Image Lab Logs 177
- User Guide 177
- 12 178
- 13 Using the Security Edition 179
- No lock indicates standard 180
- Lock indicates secure 180
- TDS_User Supervisor 181
- TDS_Tech Clinician 181
- TDS_Guest Reviewer 181
- Role Restrictions 182
- Electronic Records 183
- Unsecured Documents 184
- Secure Documents 184
- Modifying Secure Documents 185
- Signing Documents 186
- Document Logs 187
- Log file for saved image file 188
- A Maintenance 189
- A 190
- To replace the lamps 191
- Fuse Replacement 192
- B Troubleshooting 193
- B 194
- C Setting Up Users and 195
- User Authentication 196
- Group Membership Evaluation 196
- User Guide 197
- User Guide 199
- User Guide 201
- User Guide 203
- User Guide 205
- Password Security 206
- User Guide 207
- Auditing Windows Event Logs 208
- User Guide 209
- D Accessories 211
- XcitaBlue™ Conversion Screen 212
- User Guide 213
- Gel Alignment Template Kit 214
- White Light Transilluminator 215
- D 216
- User Guide 217
- Ordering Information 218
- Installation Kits 219
- Optional Filters 219
- Replacement Parts 219
- Optional Analysis Software 220
- Protein Standards 220
- Nucleic Acid Standards 221
- E Using Bio-Rad’s 223
- Stain-Free Technology 223
- Stain-Free Workflow 224
- Imaging Gels 225
- Imaging Blots 226
- F Mitsubishi P93/P95 227
- Thermal Printer 227
- G Regression Calculation 229
- Glossary 231
- 232
- Life Science 236
- Bio-Rad 236
- Laboratories, Inc 236
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